Scyl a serrata (Mud Crab) Seed Production And Its Health Management
• Researcher : Dr. Lee Seong Wei • Co- Researcher : Dr. Ikhwanuddin b. Abdullah@Polity (UMT) • Studen : Cik Syahrizawati Binti Mohd Zohri • Grant : Short Term Research Grant Abstract
The culture of mud crab, Scyl a spp, is widespread because the methods variety of farming technique exists for mud crab culture. Farming of mud crab has been progressing rapidly due to a promising market and profitability. Mud crab is recognized as a candidate species for culture in brackish water ponds and other suitable impounded brackish water environment. Recently, the demand for seafood especially shrimp, lobster and live crab for export has been increasing quite considerably. In domestic market, Scyl a serrata is much demand and fetches a good price compared to other crabs species due to its excellent meat quality.
There are three objectives of this study
• To develop the protocol for rearing of Scyl a serrata juvenile in hatchery technology. • To characterize the luminescent bacteria by isolation and identification from Scyl a serrata larval and
• To investigate the effect of antibiotic from medical plant extracts as its treatment.
The significant of this study is to develop techniques for mass production of mud crab seed. The low percentage of survival rate in larval and megalopa stages under the laboratory condition has to investigate using alternative approaches to enhance the mass production of crab seed in Malaysia. Lack of knowledge is the major problem facing this industry. Further information is needed on the breeding habits and biology, seeding or restocking techniques and effects of the crab farming on mangroves. Although there have many publications particularly in recent years on larval culture technique, only a few have focused on embryonic and larval development and also bacterial isolation and identification of Scyl a sp. The main problems in S. serrata hatchery technology seem to be bacterial or fungal losses during the zoea stages. The tendency use antibacterial and other chemical due to increasing of seed losses. Use of antimicrobial drugs, pesticides and disinfectants in aquaculture disease prevention and
growth promotion has led to the evolution of resistant strains of bacteria and questions of safety. Part of disease problem and poor water quality is still another serious bottleneck in most hatcheries and its maybe directly and indirectly associated with microbiological problems. Studies about theScyl a sp is needed that would be useful to promote the mud crab faming in Malaysia. Therefore, rational of this study is hopeful y capable to promote the growth, increasing the survival and also meet the seed production demand in commercial scale.
Barried female of Scyl a serrata was maintained under the laboratory condition. The embryonic development of every stage was observed until the eggs hatched. The larval then were rearing in tank with all the necessary needed until the completion of life cycle. In bacterial isolation and identification method, seven agar plate medium were used as follows: Tryptic Soy Agar (TSA), Mac Conkey, Thiosulphate Citrate Bile Salt (TCBS), Eosin Methylene Blue (EMB), Glutamate Starch Pseudomonas (GSP), Xylose Lysine Deoxycholate (XLD) and Baird Parker Media. The antibiotics used in Antibiotic Susceptibility Test are oxolinic acid (2 μg), ampicilin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), oleandomycin (15 μg), amoxicil in (25 μg), colistin sulphate (25 μg), sulphamethoxazole (25 μg), chloramphenicol (30 μg), doxycycline (30 μg), florfenicol (30 μg), kanamycin (30 μg), nalidixic (30 μg), novobiocin (30 μg), oxytetracycline (30 μg), tetracycline (30 μg), nitrofurantoin (50 μg), fosfomycin (50 μg) and spiramycin (100 μg) (Oxoid, England). About 30 species of plant were extracting to screening the antimicrobial activities determined by disc diffusion method. Data on survival and body measurement were analyzed using SPSS version 13.0. The data of embryonic and larval development are analyzed descriptively.
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