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Ggs_jl_008(mas)_13

GGS-JL-008-03/13
Metaphase Arresting Solution

Pack size supplied – powder to make up 100mls working solution GTIN-13: 5060174130076
Date of issue: January 2012
Revision date: March 2013
PRODUCT DESCRIPTION

PRODUCT DESCRIPTION
Metaphase Arresting Solution
Is a mixture of colchicine/vinblastine sulphate dissolved in PBS. Both compounds inhibit the formation of mitotic spindles and are used to
increase the percentage of metaphase cells for chromosome studies. Vinblastine sulphate has been found to be more effective against bone
marrows, difficult tumours and problematic tissues with low mitotic indexes, and where colchicine/colcemid appears to be ineffective.
PROCEDURE
Dissolve the contents of the supplied vial with 100ml of milli-Q or reverse osmosis filtered deionised water.
1. Use Metaphase Arresting Solution as a direct replacement for colchicine/colcemid in your harvest, keeping volume and incubation time
2. After incubation continue with your usual harvest protocols. QUALITY CONTROL All batches are tested on established bone marrow and tumour cell-lines with mitotic index and morphology scored under a compound microscope STORAGE Store at 40C for no more than 12 months. Never heat or freeze. Protect from UV light. For laboratory research use only. IDENTIFICATION OF THE SUBSTANCE AND THE COMPANY

Product: Cat# GGS-JL-008 Metaphase Arresting Solution
For laboratory and research purposes only.
Company details:
Genial Genetics
Sealand Raod Chester CH1 4QS United Kingdom COMPOSITION AND COMPONENT DATA

Proprietary product containing a mixture of colchicine and vinblastine sulphate.
HAZARDS IDENTIFICATION
WARNING: Product is toxic. Product is harmful by inhalation, skin contact and if swallowed.
FIRST-AID MEASURES

AFTER INHALATION
Remove from exposure, if difficulty in breathing seek medical advice.
AFTER EYE CONTACT
Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Seek medical advice.
Genial Genetics | P.O. Box 3751 | Chester| CH1 9UF UK Tel: +44 (0)1244 757 155 | Fax: +44 (0)1244 757 155 Page 1 of 2
GGS-JL-008-03/13
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. If ingested - Give large quantities of water. Seek medical advice.

AFTER SKIN CONTACT
Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before
reuse. Seek medical advice is skin has been severely exposed.
FIRE FIGHTING MEASURES

Not flammable, but will emit toxic fumes under fire conditions.
ACCIDENTAL RELEASE MEASURES
Avoid raising dust. Wear self-contained breathing apparatus. Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic
bag for proper disposal.
HANDLING AND STORAGE
Keep container tightly closed when not in use.
Stored in dark container at 4°C, prepared working solution is stable for 12 months.
Powdered stock product is stable at 4°C for 12 months.
EXPOSURE CONTROL AND PERSONAL PROTECTION

Only work in a Class 2 Biological Safety Cabinet or Cytotoxic Cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling.
PHYSICAL AND CHEMICAL PROPERTIES
Appearance: white/yellow powder
Boiling Point: As for water (for prepared working solution)
Vapour Pressure (pascals): As for water (for prepared working solution)
Specific Gravity: 1.2 (for prepared working solution)
Flashpoint: N/A
Flammability Limits (%): N/A
STABILITY AND REACTIVITY
Incompatible with strong oxidising agents.
TOXICOLOGICAL INFORMATION
Very toxic
Carcinogen.
Teratogen
ECOLOGICAL INFORMATION

Data not yet available
DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.
TRANSPORT INFORMATION

Not applicable.
REGULATORY INFORMATION

EC Index no: 614 – 005 – 00 – 6 - Very Toxic
R 26/ 27/ 28 – Very toxic by inhalation, in contact with skin and if swallowed
R 40 – limited evidence of a carcinogenic effect
S 36/ 37/ 39 – Wear suitable protective clothing, gloves and eye/face protection
S 7/ 47 – Keep container tightly closed and at temperature not exceeding 40C
S 41 – In case of fire and/or explosion do not breath fumes
S 56 – Dispose of this material and its container at a hazardous or special waste collection point
OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic
Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.
Genial Genetics | P.O. Box 3751 | Chester| CH1 9UF UK Tel: +44 (0)1244 757 155 | Fax: +44 (0)1244 757 155 Page 2 of 2

Source: http://www.medisa.com.tr/dosyalar/dosya-GGS_JL_008(MAS)_Metaphase%20Arresting%20Solution_13.pdf

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WELDABLE FINE-GRAINED STRUCTURAL STEEL, THERMOMECHANICALLY ROLLEDDI-MC 420 is a thermomechanically rolled, fine-grained structural steel. Due to its chemical composition, this material hasa low carbon equivalent and hence excellent weldability. It is preferentially used by the customers in constructionalsteelwork, hydraulic steelwork and mechanical engineering, where exacting demands are placed

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