Pack size supplied – powder to make up 100mls working solution
GTIN-13: 5060174130076 Date of issue: January 2012 Revision date: March 2013 PRODUCT DESCRIPTION
PRODUCT DESCRIPTION Metaphase Arresting Solution Is a mixture of colchicine/vinblastine sulphate dissolved in PBS. Both compounds inhibit the formation of mitotic spindles and are used to increase the percentage of metaphase cells for chromosome studies. Vinblastine sulphate has been found to be more effective against bone marrows, difficult tumours and problematic tissues with low mitotic indexes, and where colchicine/colcemid appears to be ineffective. PROCEDURE Dissolve the contents of the supplied vial with 100ml of milli-Q or reverse osmosis filtered deionised water. 1. Use Metaphase Arresting Solution as a direct replacement for colchicine/colcemid in your harvest, keeping volume and incubation time
2. After incubation continue with your usual harvest protocols. QUALITY CONTROL All batches are tested on established bone marrow and tumour cell-lines with mitotic index and morphology scored under a compound microscope STORAGE Store at 40C for no more than 12 months. Never heat or freeze. Protect from UV light. For laboratory research use only.
IDENTIFICATION OF THE SUBSTANCE AND THE COMPANY
Product: Cat# GGS-JL-008 Metaphase Arresting Solution For laboratory and research purposes only. Company details: Genial Genetics
Sealand Raod Chester CH1 4QS United Kingdom
COMPOSITION AND COMPONENT DATA
Proprietary product containing a mixture of colchicine and vinblastine sulphate.
HAZARDS IDENTIFICATION WARNING: Product is toxic. Product is harmful by inhalation, skin contact and if swallowed. FIRST-AID MEASURES
AFTER INHALATION Remove from exposure, if difficulty in breathing seek medical advice. AFTER EYE CONTACT Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Seek medical advice.
Genial Genetics | P.O. Box 3751 | Chester| CH1 9UF
UK Tel: +44 (0)1244 757 155 | Fax: +44 (0)1244 757 155
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GGS-JL-008-03/13 AFTER INGESTION If swallowed, wash out mouth with water provided person is conscious. If ingested - Give large quantities of water. Seek medical advice. AFTER SKIN CONTACT Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Seek medical advice is skin has been severely exposed. FIRE FIGHTING MEASURES
Not flammable, but will emit toxic fumes under fire conditions. ACCIDENTAL RELEASE MEASURES Avoid raising dust. Wear self-contained breathing apparatus. Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal. HANDLING AND STORAGE Keep container tightly closed when not in use. Stored in dark container at 4°C, prepared working solution is stable for 12 months. Powdered stock product is stable at 4°C for 12 months. EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet or Cytotoxic Cabinet. Always wear a lab coat and rubber gloves. Wash hands after handling. PHYSICAL AND CHEMICAL PROPERTIES Appearance: white/yellow powder Boiling Point: As for water (for prepared working solution) Vapour Pressure (pascals): As for water (for prepared working solution) Specific Gravity: 1.2 (for prepared working solution) Flashpoint: N/A Flammability Limits (%): N/A STABILITY AND REACTIVITY Incompatible with strong oxidising agents. TOXICOLOGICAL INFORMATION Very toxic Carcinogen. Teratogen ECOLOGICAL INFORMATION
Data not yet available DISPOSAL CONSIDERATIONS Use approved government contractor. Observe all International, National, State and Local environmental laws. TRANSPORT INFORMATION
Not applicable. REGULATORY INFORMATION
EC Index no: 614 – 005 – 00 – 6 - Very Toxic R 26/ 27/ 28 – Very toxic by inhalation, in contact with skin and if swallowed R 40 – limited evidence of a carcinogenic effect S 36/ 37/ 39 – Wear suitable protective clothing, gloves and eye/face protection S 7/ 47 – Keep container tightly closed and at temperature not exceeding 40C S 41 – In case of fire and/or explosion do not breath fumes S 56 – Dispose of this material and its container at a hazardous or special waste collection point OTHER INFORMATION The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.
Genial Genetics | P.O. Box 3751 | Chester| CH1 9UF
UK Tel: +44 (0)1244 757 155 | Fax: +44 (0)1244 757 155
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The Jerusalem Institute for Israel Studies The Center for Environmental Policy Air Pollution from Transportation Position Paper The Jerusalem Institute for Israel Studie Air Pollution from Transportation Position Paper The Arava Institute for Environmental StudiesThis book was made possible by funds granted by the Charles H. RevsonThe statements made and the views expre
WELDABLE FINE-GRAINED STRUCTURAL STEEL, THERMOMECHANICALLY ROLLEDDI-MC 420 is a thermomechanically rolled, fine-grained structural steel. Due to its chemical composition, this material hasa low carbon equivalent and hence excellent weldability. It is preferentially used by the customers in constructionalsteelwork, hydraulic steelwork and mechanical engineering, where exacting demands are placed