Faculty of Resource Science and Technology, UNIMAS
This course is a continuation from the basic course, STB1083
Biochemistry. This course is designed to cover topics in the
biochemical reactions within and between the cells that will be
describe in details in this course. Products generated from the
biosynthesis of either amino acids, protein, carbohydrate, fatty
acid or lipids are broken down for the production of energy
either in aerobic or anaerobic processes. Amongst the concepts to
be taught and discussed include a description in details of the
production of energy consists details of all the metabolic
pathways including Glycolysis, TCA cycle or Krebs cycle,
oxidative phosphorylation, photosynthesis and etc. This will be
followed by topics on mechanism of nucleotides with lectures in
biosynthesis of purine and pyrimidine. This is an advanced
biochemistry course that aims to equip students with sufficient
knowledge and information for better understanding of the
biochemical processes and metabolic pathways that involved in
(Kursus ini adalah lanjutan dari kursus asas STB1083 Biokimia.Ia direkabentuk untuk mencakupi topik-topik berkaitantindakbalas biokimia didalam dan diantara sel yang akandibincangkan secara lebih mendalam dan terperinci. Hasil akhirdari biosintesis sama ada asid amino, protein, karbohidrat, asidlemak atau lipid akan didegradasikan untuk tujuan penghasilantenaga baik secara proses aerobik mahupun anaerobik. Antarakonsep-konsep yang akan dipelajari dan dibincangkantermasuklah kesemua kitaran metobolik penting di dalampenghasilan tenaga seperti glikolisis, kitaran asid sitrik ataukitaran Kreb, fosforilasi oksidatif, fotosintesis dan sebagainya.Ini akan seterusnya diikuti dengan topik mekanisma nukleotidaFaculty of Resource Science and Technology, UNIMASdengan syarahan dalam biosintesis purin dan pirimidin. Kursusini adalah kursus biokimia lanjutan yang bertujuan untukmelengkapkan pelajar dengan pengetahuan untuk lebihmemahami tentang proses biokimia dan kitaran metabolik yang
By the end of this course, students will be able to:
Interpret and compare the chemical processes and the
interactions involved between the living cells in the
production of energy and chemicals through the various
Explain the chemical processes and the interactions
involved between the living cells in the production of
energy and chemicals through the various metabolic
120 Hour/s
-Glycolysis: details on the degradation including
-Alcoholic fermentation and Pentose Phosphate
alternate path for glucose oxidation.
-TCA (Krebs Cycle) Cycle: detailed description of
and the production of asetyl-S-CoA and the cycle
-Balancing reaction and regulation: anaplerotic or
Faculty of Resource Science and Technology, UNIMAS
regulation of glycolysis and TCA cycle.
-Description of the electron transport system and
phosphorylation intermediates and stucture
-ATP synthesis and chemiosmotic mechanism.
-Photosynthesis: the overall reactions (cyclic and
-The light reaction and generation of ATP and
-The dark reactions in both C3 and C4 plants,
(glyoxcylate cycle), glycogen synthesis and
-Nitrogen assimilation involving glitamine and
of amino acid synthesis: ser/gly, val, asp/thr/ile.
-Amino acid oxidation and urea production including
significant and mechanism, oxidative deamination
glutamine and its function as nitrogen transporter.
-Nucleotide synthesis and metabolism including:
synthesis by the de novo and salvage pathways,
synthesis, nucleotide kinases, pyrophosphorylases,
Faculty of Resource Science and Technology, UNIMAS
deoxynucleotide synthesis including dTMP.
-Application of biochemistry in the field of medicine,
-Information of biochemistry used and applied in
forensic science, manufacture of industrial/cosmetic
agricultural technology development and products,
added products, wastewater treatments and etc.
-Laboratory demonstration of basic biochemistry
preparation of buffers, agarose gel electrophoresis,
purification and basic enzyme kinetics studies.
-Industrially important enzymes: production, final
-Technology of enzyme hybrids and modification,
immobilization, secondary metabolite biosynthesis
and protein methods such as LC MS/MS and
Faculty of Resource Science and Technology, UNIMAS
Mathews, C. K., Kensal, E., van Holde and Ahern, K.
G.(2000). Biochemistry. 3rd Edition. USA :
Berg, J. M., Tymoczko, J. T. and Stryer L. (2007).
Biochemistry. 6th Edition. USA: W. H. Freeman and
Nelson, D. C., and Cox, M. M. (2004). Lehninger:
Principles of Biochemistry. 4th Edition USA: W. H.
Voet, D. and Voet, J. G. (2005). Biochemistry. 3rd Edition.
Horton, R. H., Moran, L. A., Ochs, R. S., Rawn, J. D. and
Scrimgeour K. G. (2006). Principle of Biochemistry 4nd
New Jersey, USA: Prentice-Hall International Inc.
Cambell, M. K. and Farrell, S. O. (2006). Biochemistry.
Fifth Edition. USA: Thompson Brooks/Cole. Last updated: May 3, 2011, Tue, 4:25 PM
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