Catalog Number: 100535, 194788D-Cycloserine
R (+)-4-amino-3-isoxazolidinone: D-4-amino-3-isoxazolidinone
White to off white powder
Soluble in water (up to 100 mg/ml - clear, colorless to faint yel ow solution), 96% ethanol (1 in 50 parts ethanol); slightly
soluble in methanol or propylene glycol; practical y insoluble in chloroform and ether. It is recommened to prepare solutions fresh for
each use (neutral and acidic solutions are very unstable); however, aqueous solutions buffered to pH 10 with sodium carbonate may
be stored for up to one week if stored at 2-8°C.
Mode of Resistance:
D-Ala transport interference.
Studies in vitro show no suppression of growth in cultures made in media containing D-alanine which appears to block the
antibacterial action of D-cycloserine.5
A structural analog of D-Alanine. A partial agonist at the glycine modulatory site of NMDA receptors11,16,19; enhances
learning and memory in several models of cognitive deficit4,10,11,12,14,15,16,18; anticonvulsant (at high doses).2,20 Antibiotic against
gram-negative bacteria that acts by inhibiting the synthesis of the cel wal (D-Ala peptide bond formation).13
D-Cycloserine has antibiotic activity in vitro against growth phase gram-negative bacteria including Escherichia coli
(workingconcentration of approximately 200 ug/ml)13, strains of Staphylococcus aureus
species and Chlamydia
,5 and somemycobacteria including Mycobacterium tuberculosis
. The minimum inhibitory concentrations (MIC) in vitro for M. tuberculosis
rangefrom 5-20 ug/ml.
Typical Concentration for Use:
100-200 ug/ml. Prepare just before use using 0.1 M sodium phosphate buffer, pH 8.0.
1. Merck Index, 12th Ed
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Academic Press; New York, p. 567 (1989).
4. Flood, J.F., et al., Eur. J. Pharmacol.
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5. Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th Ed.
, Hardman, J.G., et al. (eds.), p. 1164, McGraw-
6. Jain, M.K., Handbook of Enzyme Inhibitors
, John Wiley & Sons; New York, p. 112 (1982).
7. Jones, L.R., Anal. Chem.
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, 39 (1956).
8. Kuehl, F.A., Jr., et al., J. Am. Chem. Soc.
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receptor blockade in rats." Life Sci.
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11. Ohno, M. and Watanabe, S., Eur. J. Pharmacol.
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, 267 (1996).
12. Pussinen, R. and Sirvio, J., "Effects of D-cycloserine, a positive modulator of N-methyl-D-aspartate receptors, and ST 587, a
putative alpha-1 adrenergic agonist, individual y and in combination, on the non-delayed and delayed foraging behaviour of rats
assessed in the radial arm maze." J. Psychopharmacol.
, v. 13
, 171 (1999).
13. Raleigh, E.A., et al., "Selected Topics from Classical Bacterial Genetics" in Short Protocols in Molecular Biology, 4th Ed.
Ausubel, F.M., et al. (eds.), Unit 1.4, p. 1-9 (John Wiley & Sons, Inc. New York (1999).
14. Schneider, J.S., et al., "Effects of the partial glycine agonist D-cycloserine on cognitive functioning in chronic low dose MPTP-
treated monkeys." Brain Res.
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, 190-194 (2000).
15. Schuster, G.M. and Schmidt, W.J., Eur. J. Pharmacol.
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, 97 (1992).
16. Sirvio, J., et al., Neurosci. Lett.
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, 215 (1992).
17. Stammer, C.H., et al., J. Am. Chem. Soc.
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, 2346 (1955).
18. Temple, M.D. and Hamm, R.J., Brain Res.
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19. Watson, et al., "D-Cycloserine acts as a partial agonist at the glycine modulatory site of the NMDA receptor expressed in
Xenopus oocytes." Brain Res.
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, 158-160 (1990).
20. Wlaz, P., et al., "Influence of D-cycloserine on the anticonvulsant activity of phenytoin and carbamazepine against
electroconvulsions in mice." Epilepsia
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