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Microsoft word - sbpmat_gg

Conformation of therapeutic lens, from bacterial cellulose
with ciprofloxacin incorporation for medical application
C. M. Caliri1, R. Marchetto1, W. R. Lustri,Y. Messaddeq. 1Instituto de Química – UNESP, Araraquara, SP, Brazil Currently, several methods have been proposed for the controlled release of ophthalmic drugs using soft contact lenses. However, the current systems do not promote a controlled release for more than 24 hours. Therefore, the bacterial cellulose (BC), a hydrogel polymer easily adjustable to medical applications and subjected to incorporation of therapeutic agents, becomes a material of great interest for such therapeutic purpose. The focuses of this work were the conformation of hydrophilic therapeutic lenses from bacterial cellulose, the development of a controlled release system of ciprofloxacin antibiotic as well as to carry out a quantification of their release and to analyse their antimicrobial activity in vitro. The techniques involved in the characterization of these lenses were: scanning electronic microscopy (SEM); thermogravimetry (TG); differential scanning calorimetry (DSC); X-ray difratometry (XRD) and swelling. The bacterial sensitivity test was conducted with strains of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, using paper disks soaked in solutions of ciprofloxacin, taken every six hours from the drug release tests. To accomplish this, the strains were grown on glass plates containing BHI (Brain Heart Infusion) for 18 hours. After this period, the soaked disks, with different ciprofloxacin concentrations, were inserted on dry plates. The antimicrobial tests showed growth inhibition of Staphylococcus aureus and Escherichia coli bacteria. Moreover, the pharmacological activity of the ciprofloxacin was not altered with the conformation process of the BC membrane. It can be
concluded that the BC lenses are a potent carrier of ciprofloxacin, allowing its release for a period
of up to 48 hours.

Keywords: bacterial cellulose; therapeutic lenses; controlled release

This work is supported by FAPESP

[1] A.Wojciechczaj, A. Krystynowicza, S. Bieleckia, R. M. Brown. Biomaterials 27, 145 (2006).
[2] L. M. Cabral, S. J. L. Ribeiro, E. Pecoraro, Y. Messaddeq. In:Celulose bacteriana – propriedades e aplicações na prática clínica. Cap. 115, 2006, v. 1, p. 901-910. [3] CASTRO, C.; EVORA,M.; BARO, M,; SORIANO,I.; SANCHEZ,E. Two-month ciprofloxacin implants for multibacterial bone infections. Eur. J. Pharm. Biopharm.,v.60, p.401-406,2005.
[4] SULEWSKI, M. E.; KRECHER, G. P.; GOTTSCH, J. D.; STARK, W.J. Use of disposable contact lens as a bandage contact lens.Arch. Ophthalmol. v. 109, p. 318-320, 1991.
[5] BECHARA, S. J.; KARA-JOSÉ, N. Lentes de contato terapêuticas. 1ª ed. São Paulo:Roca L,
of transcorneal iontophoresis to corneal collagen shields. Int J Pharm, v.9, p.123:173, 1995.
[6] MACEDO FIHLO; LAGO. A.; DUARTE, K.; JUNG, L.S.; LIMA, A.L.; FREITAS, D. Superficialcorneal foreign body: laboratory and epidemiologic aspects. Arq Bras Oftalmol., v.6,

Source: http://sbpmat.org.br/9encontro/especific_files/papers/A1011.pdf

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